TACR1 antibody (Internal Region)

Catalog No. ABIN190795

Product Details anti-TACR1 Antibody

Target: TACR1 (Tachykinin Receptor 1 (TACR1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This TACR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: TACR1

Sequence: EIPGDSSDRY HEQ

Specificity: This antibody is expected to recognise both reported isoforms (NP_001049.1 and NP_056542.1).

Cross-Reactivity: Dog, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-EIPGDSSDRYHEQ, from the internal region of the protein sequence according to NP_001049.1*, NP_056542.1*.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 48 kDa band observed in lysates of cell lines HeLa and Neuro2a (calculated MW of 46.3 kDa according to Human NP_001049.1 and Mouse NP_033339.2 ). Recommended concentration: 0.3-1 μg/mL.. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Expression of the protein seen in the plasma membrane and nucleus of Neuro2a cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TACR1

Target: TACR1 (Tachykinin Receptor 1 (TACR1))

Alternative Name: TACR1 (TACR1 Products)

Synonyms: NK1R antibody, NKIR antibody, SPR antibody, TAC1R antibody, Nk1r antibody, Spr antibody, Tac1r antibody, nk1R antibody, Tacr1 antibody, ASPR antibody, tachykinin receptor 1 antibody, TACR1 antibody, Tacr1 antibody

Background: TACR1, tachykinin receptor 1, NK1R, neurokinin 1 receptor, NKIR, SPR, TAC1R , NK-1 receptor, Tachykinin receptor 1 (substance P receptor,neurokinin-1 receptor), neurokinin 1 receptor, tachykinin 1 receptor (substance P receptor, neurokinin 1 receptor)

Gene ID: 6869, 21336, 24807

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration