GABPB2 antibody (Internal Region)

Catalog No. ABIN190778

Product Details anti-GABPB2 Antibody

Target: GABPB2 (GA-Binding Protein Subunit beta-2 (GABPB2))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GABPB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: GABPB2

Sequence: SSENSSKATD ETG

Cross-Reactivity: Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-SSENSSKATDETG, from the internal region of the protein sequence according to NP_005245.2, NP_002032.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Tonsil and Spleen. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 45+38 kDa bands observed in lysates of cell line HeLa (calculated MW of 42.5 kDa according to NP_005245.2, NP_001307844.1 and of 38.1 kDa according to NP_002032.2). An additional band of unknown identity was also consistently observed

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 1

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GABPB2

Target: GABPB2 (GA-Binding Protein Subunit beta-2 (GABPB2))

Alternative Name: GABPB2 (GABPB2 Products)

Background: GABPB2, GA binding protein transcription factor, beta subunit 2, BABPB2, E4TF1, E4TF1-47, E4TF1-53, E4TF1B, GABPB, GABPB1, NRF2B1, NRF2B2, GA binding protein transcription factor, beta subunit 1, 53 kDa, GA binding protein transcription factor, beta subuni

Gene ID: 2553

NCBI Accession: NP_005245, NP_002032, NP_001307844