Glucose-6-Phosphate Dehydrogenase antibody (AA 308-320)

Catalog No. ABIN190766

Product Details anti-Glucose-6-Phosphate Dehydrogenase Antibody

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Binding Specificity: AA 308-320

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Glucose-6-Phosphate Dehydrogenase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: G6PD (aa 308 - 320)

Sequence: STNSDDVRDE KVK

Specificity: This antibody is expected to recognise both reported isoforms (NP_000393.4 and NP_001035810.1).

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-STNSDDVRDEKVK, from the internal region of the protein sequence according to NP_000393.4 , NP_001035810.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Testis and Spleen. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 55-60 kDa double band observed in Human Testis, Thyroid and Tonsil lysates (calculated MW of 62.5 kDa according to NP_000393.4 and 59.3 kDa accordiong to NP_001035810.1). Recommended concentration: 0.03-0.1 μg/mL. Primary incubation was

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Glucose-6-Phosphate Dehydrogenase antibody (ABIN190766)

Hwang, Mruk, Rahighi, Raub, Chen, Dorn, Horikoshi, Wakatsuki, Chen, Mochly-Rosen: "Correcting glucose-6-phosphate dehydrogenase deficiency with a small-molecule activator." in: Nature communications, Vol. 9, Issue 1, pp. 4045, (2019) (PubMed).

Target Details for Glucose-6-Phosphate Dehydrogenase

Target: Glucose-6-Phosphate Dehydrogenase (G6PD)

Alternative Name: G6PD (G6PD Products)

Background: G6PD, glucose-6-phosphate dehydrogenase, G6PD1, glucose-6-phosphate 1-dehydrogenase, glucose-6-phosphate dehydrogenase, G6PD

Gene ID: 2539, 14381, 24377

NCBI Accession: NP_000393, NP_001035810

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones