Rpa12p (RPA12) antibody
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- Target
- Rpa12p (RPA12)
- Reactivity
- Arabidopsis thaliana
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Western Blotting (WB)
- Cross-Reactivity (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
- Predicted Reactivity
- Arabidopsis thaliana
- Characteristics
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Expected / apparent Molecular Weight of the Antigene: 13.6
This product has previously been labelled as anti-At3g25940 transcription factorS-II (TFIIS) domain-containing protein. - Purification
- serum
- Immunogen
- KLH-conjugated peptide derived from the Arabidopsis thaliana A12.2 (At3g25940) protein sequence. This sequence is only weakly conserved in other eukaryotic sequences available in the databases.
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- Application Notes
- 1 : 2 000 with standard ECL (WB)
- Comment
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This antibody is specific for A12.2 subunit of RNA polymerase I but NOT RNA polymerase II or IV from Arabidopsis thaliana
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 143 μL of sterile water
- Buffer
- PBS pH 7.4
- Handling Advice
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - Storage
- -20 °C
- Storage Comment
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- Target
- Rpa12p (RPA12)
- Alternative Name
- A12.2
- Synonyms
- RRN4 antibody, DNA-directed RNA polymerase I subunit RPA12 antibody, RPA12 antibody
- Background
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AGI Code: At3g25940
RNA polymerase I is a nuclear located DNA-dependent enzyme involved in RNA elongation and regulation of transcription. In yeast subunit A12.2 has been described as homologous to the Rpb9 subunit from polymerase II. - Molecular Weight
- 13.6
- UniProt
- Q9LU97
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