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Chloroplastic Lipoxygenase antibody (AA 257-450)

LoxC Reactivity: Arabidopsis thaliana, Grapes WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN190712
  • Target
    Chloroplastic Lipoxygenase (LoxC)
    Binding Specificity
    AA 257-450
    Reactivity
    Arabidopsis thaliana, Grapes
    Host
    Rabbit
    Clonality
    Polyclonal
    Application
    Western Blotting (WB)
    Cross-Reactivity (Details)
    Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
    Predicted Reactivity
    dicots including: Brassica napus
    Characteristics
    Expected / apparent Molecular Weight of the Antigene: 102 / 97 kDa
    Purification
    serum
    Immunogen
    recombinant Arabidopsis thaliana protein, loop (aa 257-450) P38418, At3g45140
  • Application Notes
    1: 50 000 with alkaline phosphatase (WB)
    Comment

    a weak band at around 84 kDa is detected as a probable result of cross-reaction with another lipoxygenase

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 100 μL of sterile water.
    Handling Advice
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Target
    Chloroplastic Lipoxygenase (LoxC)
    Alternative Name
    Chloroplastic lipoxygenase (LOX-C)
    Background
    AGI Code: At3g45140
    Lipoxygenases (LOXs, EC 1.13.11.12, synonym: lipoxydases) are a family of enzymes that catalyze oxygenation of polyunsaturated fatty acids (PUFAs) into lipidhydroperoxides (LOOHs) involved in responses to stresses. LOXs has been found to play a role in plant growth and development, senescence as well as can be activated in response to environamental stress (drought, heavy metals). Alternative name of the antigen: Lipoxygenase 2
    Molecular Weight
    expected: 102 kDa, apparent: 97 kDa
    UniProt
    P38418
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