GJC2 antibody (C-Term)

Catalog No. ABIN1898541

Product Details anti-GJC2 Antibody

Target: GJC2 (Gap Junction Protein, gamma 2, 47kDa (GJC2))

Binding Specificity: C-Term

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This GJC2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Specificity: Reacts with the mouse Connexin 47 protein. On Western blots of mouse thalamus homogenates and Connexin 47-transfected HeLa cells, it identified a band at ~47kD. Reactivity was confirmed in Western blots with mouse thalamus lysates. Mouse Connexin 47-transfected HeLa cells were used in both Immunoprecipitation and Western blot assays. This antibody was also used in immunofluorescence assays using paraformaldehyde-fixed mouse brain. The expected staining pattern is punctate labeling of the oligodendrocytes in the brain.

Purification: Protein A purified

Immunogen: Synthetic peptide derived from the C-terminal region of the mouse Connexin 47 protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IF (1 - 3 μg/mL), IP (5 - 10 μg/mL), WB (1 - 3 μg/mL)

Usage: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation. Immunofluorescence: 1-3 μg/mL. Western Blot: 1-3 μg/mL. Immunoprecipitation: 5-10 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for GJC2

Target: GJC2 (Gap Junction Protein, gamma 2, 47kDa (GJC2))

Alternative Name: GJA12 / CX47 / Connexin 47 (GJC2 Products)

Background: Name/Gene ID: GJC2
Subfamily: Connexin
Family: Ion Channel

Synonyms: GJC2, Cx47, Connexin-46.6, Connexin-47, CX46.6, Gap junction protein alpha 12, GJA12, Gap junction gamma-2 protein, LMPH1C, Gap junction alpha-12 protein, HLD2, PMLDAR, SPG44

Gene ID: 57165

UniProt: Q5T442