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PCNA antibody (C-Term)

Name: PCNA antibody (C-Term)
SKU: ABIN1882273
Price: 451 USD
Availability: InStock

PCNA Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Mouse Monoclonal 1157CT1-1-3 unconjugated

All lanes : Anti-PCNA Antibody (C-term) at 1:2000 dilution Lane 1: C2C12 whole cell lysate Lane 2: C6 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: whole cell lysate Lane 5: MCF-7 whole cell lysate Lysates/proteins at 20 μg per lane. (PCNA antibody (C-Term))

Immunohistochemical analysis of paraffin-embedded H. (PCNA antibody (C-Term))

IHC (p)

Immunohistochemical analysis of paraffin-embedded Human Ovarian cancer section using Pink1 (ABIN1882273 and ABIN2838445). (PCNA antibody (C-Term))

IHC

IHC (p)

4 images

(1 reference)

Catalog No. ABIN1882273

Datasheet as PDF

Target See all PCNA Antibodies

PCNA (Proliferating Cell Nuclear Antigen (PCNA))
Binding Specificity
16

16

16

15

11

11

8

6

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 236-261, C-Term
Reactivity
206

149

148

54

28

27

21

19

19

18

17

14

11

10

6

6

5

5

4

4

4

3

3

2

2

2

2

2

1

1

1

1

1
Human, Mouse, Rat
Host
130

89

3
Mouse
Compatible Secondaries
Clonality
129

92

1
Monoclonal
Conjugate
108

16

13

8

5

5

5

5

5

5

5

5

5

5

5

4

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This PCNA antibody is un-conjugated
Application
187

68

62

60

51

47

45

40

28

15

15

11

5

4

3

2

2

2

2

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity

B, Ha, Pr

Purification

This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen

This PCNA antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 236-261 amino acids from the C-terminal region of human PCNA.

Clone

1157CT1-1-3

Isotype

IgG1

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PCNA Reactivity: Human WB, ELISA, IF, IP Host: Mouse Monoclonal 1G7 unconjugated

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PCNA Reactivity: Human WB, ELISA Host: Mouse Monoclonal S1 unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibody
PCNA Reactivity: Human WB, IHC, IF, IP Host: Rabbit Polyclonal unconjugated

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PCNA Reactivity: Human, Mouse, Rat, Chicken WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

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PCNA Reactivity: Human, Mouse, Rat, Pig, Rabbit, Dog, Rhesus Monkey WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Proliferating Cell Nuclear Antigen (PCNA) antibody
PCNA Reactivity: Human WB, IHC, IHC (p) Host: Mouse Monoclonal 1H10 unconjugated

Application Notes

WB: 1:2000. IHC: 1:2000. IHC-P: 1:25. IHC-P: 1:25

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Expiry Date

6 months
Deloukas, Matthews, Ashurst, Burton, Gilbert, Jones, Stavrides, Almeida, Babbage, Bagguley, Bailey, Barlow, Bates, Beard, Beare, Beasley, Bird, Blakey, Bridgeman, Brown, Buck, Burrill, Butler, Carder et al.: "The DNA sequence and comparative analysis of human chromosome 20. ..." in: Nature, Vol. 414, Issue 6866, pp. 865-71, (2002) (PubMed).
Target

PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name

PCNA (PCNA Products)

Background

This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.

Molecular Weight

28769

NCBI Accession

NP_002583, NP_872590

UniProt

P12004

Pathways

Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy