ERK1/2 antibody

Catalog No. ABIN1882239

Product Details anti-ERK1/2 Antibody

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ERK1/2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: Purified His-tagged Erk1/2 protein was used to produced this monoclonal antibody.

Clone: 784CT7-6-3

Isotype: IgG2a

Application Details

Application Notes: WB: 1:4000. FC: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-ERK1/2 antibody (ABIN1882239)

Hu, Zhou, Zhao, Wu: "Integrin α6/Akt/Erk signaling is essential for human breast cancer resistance to radiotherapy." in: Scientific reports, Vol. 6, pp. 33376, (2018) (PubMed).

Wang, Zhao, Li, Chen, Guan, Chen, Guan: "Neuroprotective Effect and Mechanism of Thiazolidinedione on Dopaminergic NeuronsIn VivoandIn Vitroin Parkinson's Disease." in: PPAR research, Vol. 2017, pp. 4089214, (2017) (PubMed).

Guo, Meng, Bai, Wei, Shi, Davis, Mao: "Prostaglandin F2? induces expression of activating transcription factor 3 (ATF3) and activates MAPK signaling in the rat corpus luteum." in: Acta histochemica, Vol. 117, Issue 2, pp. 211-8, (2015) (PubMed).

Zhao, Ye, Wu, Liu, Yang, Gao, Chen, Zhang, Yang, Li: "Sp1-CD147 positive feedback loop promotes the invasion ability of ovarian cancer." in: Oncology reports, Vol. 34, Issue 1, pp. 67-76, (2015) (PubMed).

Rönnstrand: "Signal transduction via the stem cell factor receptor/c-Kit." in: Cellular and molecular life sciences : CMLS, Vol. 61, Issue 19-20, pp. 2535-48, (2004) (PubMed).

Sano, Liu, Lane, Piletz, Lienhard: "Insulin receptor substrate 4 associates with the protein IRAS." in: The Journal of biological chemistry, Vol. 277, Issue 22, pp. 19439-47, (2002) (PubMed).

Target Details for ERK1/2

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Alternative Name: Erk1/2 (MAPK1/3 Products)

Synonyms: erk1/2 antibody, mitogen-activated protein kinase antibody, erk1/2 antibody

Background: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The the MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.

Molecular Weight: 43136

UniProt: P27361