PCK1 antibody (C-Term)

Catalog No. ABIN1882180

Product Details anti-PCK1 Antibody

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Binding Specificity: AA 592-622, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This PCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 592-622 amino acids from the C-terminal region of human PCK1.

Clone: RB03646

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:1000. WB: 1:1000. WB: 1:2000. IHC-P-Leica: 1:250

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-PCK1 antibody (ABIN1882180)

Gummadi, Taylor, Curthoys: "Concurrent binding and modifications of AUF1 and HuR mediate the pH-responsive stabilization of phosphoenolpyruvate carboxykinase mRNA in kidney cells." in: American journal of physiology. Renal physiology, Vol. 303, Issue 11, pp. F1545-54, (2012) (PubMed).

Berger, Lindh, Wierup, Zmuda-Trzebiatowska, Lindqvist, Manganiello, Degerman: "Phosphodiesterase 3B is localized in caveolae and smooth ER in mouse hepatocytes and is important in the regulation of glucose and lipid metabolism." in: PLoS ONE, Vol. 4, Issue 3, pp. e4671, (2009) (PubMed).

Franckhauser, Muñoz, Elias, Ferre, Bosch: "Adipose overexpression of phosphoenolpyruvate carboxykinase leads to high susceptibility to diet-induced insulin resistance and obesity." in: Diabetes, Vol. 55, Issue 2, pp. 273-80, (2006) (PubMed).

Dunten, Belunis, Crowther, Hollfelder, Kammlott, Levin, Michel, Ramsey, Swain, Weber, Wertheimer: "Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site." in: Journal of molecular biology, Vol. 316, Issue 2, pp. 257-64, (2002) (PubMed).

Strausberg, Feingold, Grouse, Derge, Klausner, Collins, Wagner, Shenmen, Schuler, Altschul, Zeeberg, Buetow, Schaefer, Bhat, Hopkins, Jordan, Moore, Max, Wang, Hsieh, Diatchenko, Marusina, Farmer et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, (2002) (PubMed).

Deloukas, Matthews, Ashurst, Burton, Gilbert, Jones, Stavrides, Almeida, Babbage, Bagguley, Bailey, Barlow, Bates, Beard, Beare, Beasley, Bird, Blakey, Bridgeman, Brown, Buck, Burrill, Butler, Carder et al.: "The DNA sequence and comparative analysis of human chromosome 20. ..." in: Nature, Vol. 414, Issue 6866, pp. 865-71, (2002) (PubMed).

OBrien, Printz, Halmi, Tiesinga, Granner: "Structural and functional analysis of the human phosphoenolpyruvate carboxykinase gene promoter." in: Biochimica et biophysica acta, Vol. 1264, Issue 3, pp. 284-8, (1996) (PubMed).

Ting, Burgess, Chamberlain, Keith, Falls, Meisler: "Phosphoenolpyruvate carboxykinase (GTP): characterization of the human PCK1 gene and localization distal to MODY on chromosome 20." in: Genomics, Vol. 16, Issue 3, pp. 698-706, (1993) (PubMed).

Target Details for PCK1

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Alternative Name: PCK1 (PCK1 Products)

Background: This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme of the encoded protein also has been characterized.

Molecular Weight: 69195

NCBI Accession: NP_002582

UniProt: P35558

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis