JNK2 antibody (C-Term)

Catalog No. ABIN1882172

Product Details anti-JNK2 Antibody

Target: JNK2 (MAPK9) (Mitogen-Activated Protein Kinase 9 (MAPK9))

Binding Specificity: AA 349-379, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JNK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This JNK2 (MAPK9) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 349-379 amino acids from the C-terminal region of human JNK2 (MAPK9).

Clone: RB2510

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-JNK2 antibody (ABIN1882172)

Tetsunaga, Nishida, Furumatsu, Naruse, Hirohata, Yoshida, Saito, Ozaki: "Regulation of mechanical stress-induced MMP-13 and ADAMTS-5 expression by RUNX-2 transcriptional factor in SW1353 chondrocyte-like cells." in: Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society, Vol. 19, Issue 2, pp. 222-32, (2011) (PubMed).

Gupta, Barrett, Whitmarsh, Cavanagh, Sluss, Dérijard, Davis: "Selective interaction of JNK protein kinase isoforms with transcription factors." in: The EMBO journal, Vol. 15, Issue 11, pp. 2760-70, (1996) (PubMed).

Kallunki, Su, Tsigelny, Sluss, Dérijard, Moore, Davis, Karin: "JNK2 contains a specificity-determining region responsible for efficient c-Jun binding and phosphorylation." in: Genes & development, Vol. 8, Issue 24, pp. 2996-3007, (1995) (PubMed).

Sluss, Barrett, Dérijard, Davis: "Signal transduction by tumor necrosis factor mediated by JNK protein kinases." in: Molecular and cellular biology, Vol. 14, Issue 12, pp. 8376-84, (1994) (PubMed).

Target Details for JNK2

Target: JNK2 (MAPK9) (Mitogen-Activated Protein Kinase 9 (MAPK9))

Alternative Name: JNK2 (MAPK9) (MAPK9 Products)

Synonyms: MAPK9 antibody, JNK-55 antibody, JNK2 antibody, JNK2A antibody, JNK2ALPHA antibody, JNK2B antibody, JNK2BETA antibody, PRKM9 antibody, SAPK antibody, SAPK1a antibody, p54a antibody, p54aSAPK antibody, AI851083 antibody, Prkm9 antibody, JNK2-ALPHA1 antibody, MAP kinase 9 antibody, mitogen-activated protein kinase 9 antibody, MAP kinase 9 antibody, MAPK9 antibody, Mapk9 antibody, MPK9 antibody

Background: JNK2 responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. JNK2 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it. JNK2 is activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. It is inhibited by dual specificity phosphatases, such as DUSP1. The protein has been shown to bind to at least three scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2 and MAPK8IP3/JIP-3/JSAP1. These proteins also bind other components of the JNK signaling pathway

Molecular Weight: 48139

NCBI Accession: NP_001128516, NP_002743, NP_620707, NP_620708, NP_620709

UniProt: P45984

Pathways: MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, S100 Proteins