ATG3 antibody (C-Term)

Catalog No. ABIN1882157

Product Details anti-ATG3 Antibody

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Binding Specificity: AA 168-197, C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG3 antibody is un-conjugated

Application: Western Blotting (WB)

Predicted Reactivity: B

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ATG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-197 amino acids from the C-terminal region of human ATG3.

Clone: RB7459

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-ATG3 antibody (ABIN1882157)

Radoshevich, Murrow, Chen, Fernandez, Roy, Fung, Debnath: "ATG12 conjugation to ATG3 regulates mitochondrial homeostasis and cell death." in: Cell, Vol. 142, Issue 4, pp. 590-600, (2010) (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, (2004) (PubMed).

Target Details for ATG3

Target: ATG3 (ATG3 Autophagy Related 3 (ATG3))

Alternative Name: ATG3 (ATG3 Products)

Synonyms: atg3 antibody, im:6910145 antibody, wu:fb15g12 antibody, zgc:64156 antibody, APG3 antibody, APG3-LIKE antibody, APG3L antibody, PC3-96 antibody, 2610016C12Rik antibody, Apg3l antibody, Atg3l antibody, PIG-1 antibody, Pig1 antibody, ATATG3 antibody, K11J9.1 antibody, K11J9_1 antibody, autophagy related 3 antibody, autophagy 3 (APG3) antibody, similar to S. cerevisiae ATG3 (YNR007C) part of ubiquitin-like system that mediates Atg8-phosphatidylethanolamine conjugation required for protein-vacuolar targeting and autophagy antibody, atg3 antibody, ATG3 antibody, Atg3 antibody

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE). APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3. Formation of the PE conjugate is essential for autophagy.

Molecular Weight: 35864

NCBI Accession: NP_001265641, NP_071933

UniProt: Q9NT62

Pathways: Autophagy