YWHAB antibody (N-Term)

Catalog No. ABIN1882154

Product Details anti-YWHAB Antibody

Target: YWHAB (14-3-3 alpha + beta (YWHAB))

Binding Specificity: AA 56-85, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YWHAB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Predicted Reactivity: B, Pr, M, Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This YWHAB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-85 amino acids from the N-terminal region of human YWHAB.

Clone: RB4062

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-YWHAB antibody (ABIN1882154)

Tang, Lv, Sun, Han, Zhou: "14-3-3β Promotes Migration and Invasion of Human Hepatocellular Carcinoma Cells by Modulating Expression of MMP2 and MMP9 through PI3K/Akt/NF-κB Pathway." in: PLoS ONE, Vol. 11, Issue 1, pp. e0146070, (2016) (PubMed).

Yang, Thein, Wang, Bi, Ericksen, Xu, Han: "BSCL2/seipin regulates adipogenesis through actin cytoskeleton remodelling." in: Human molecular genetics, Vol. 23, Issue 2, pp. 502-13, (2013) (PubMed).

Yue, Song, Ma, Feng, Guan, Wu, Yang, Jiang, Liu, Cui, Guo: "Effects of triterpenes from Ganoderma lucidum on protein expression profile of HeLa cells." in: Phytomedicine : international journal of phytotherapy and phytopharmacology, Vol. 17, Issue 8-9, pp. 606-13, (2010) (PubMed).

Komori, Ishizawa, Arai, Hirose, Mizutani, Oda: "Immunoexpression of 14-3-3 proteins in glial cytoplasmic inclusions of multiple system atrophy." in: Acta neuropathologica, Vol. 106, Issue 1, pp. 66-70, (2003) (PubMed).

Cavet, Lehoux, Berk: "14-3-3beta is a p90 ribosomal S6 kinase (RSK) isoform 1-binding protein that negatively regulates RSK kinase activity." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 18376-83, (2003) (PubMed).

Li, Inoki, Vacratsis, Guan: "The p38 and MK2 kinase cascade phosphorylates tuberin, the tuberous sclerosis 2 gene product, and enhances its interaction with 14-3-3." in: The Journal of biological chemistry, Vol. 278, Issue 16, pp. 13663-71, (2003) (PubMed).

Shumway, Li, Xiong: "14-3-3beta binds to and negatively regulates the tuberous sclerosis complex 2 (TSC2) tumor suppressor gene product, tuberin." in: The Journal of biological chemistry, Vol. 278, Issue 4, pp. 2089-92, (2003) (PubMed).

Parvaresch, Yesilkaya, Baer, Al-Hasani, Klein: "14-3-3 binding to the IGF-1 receptor is mediated by serine autophosphorylation." in: FEBS letters, Vol. 532, Issue 3, pp. 357-62, (2002) (PubMed).

Target Details for YWHAB

Target: YWHAB (14-3-3 alpha + beta (YWHAB))

Alternative Name: YWHAB (YWHAB Products)

Synonyms: GW128 antibody, HS1 antibody, KCIP-1 antibody, YWHAA antibody, 1300003C17Rik antibody, wu:fb22g04 antibody, wu:fr86a12 antibody, ywhab antibody, ywhab2 antibody, zgc:64092 antibody, 14-3-3beta antibody, fb80c08 antibody, fk41f08 antibody, wu:fb80c08 antibody, wu:fd11d01 antibody, wu:fk41f08 antibody, ywhab1 antibody, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta antibody, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide antibody, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide b antibody, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta antibody, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta S homeolog antibody, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta L homeolog antibody, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide a antibody, YWHAB antibody, Ywhab antibody, ywhabb antibody, ywhab.S antibody, ywhab.L antibody, ywhaba antibody

Background: YWHAB belongs to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for the gene. Both variants encode the same protein, however, they are differentially expressed in hematopoietic cells.

Molecular Weight: 28082

NCBI Accession: NP_003395, NP_647539

UniProt: P31946

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, Maintenance of Protein Location