Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Predicted Reactivity
B
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen
This UBE2L3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-153 amino acids from the C-terminal region of human UBE2L3.
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Expiry Date
6 months
Moynihan, Cole, Dunham, ONeil, Markham, Robinson: "Fine-mapping, genomic organization, and transcript analysis of the human ubiquitin-conjugating enzyme gene UBE2L3." in: Genomics, Vol. 51, Issue 1, pp. 124-7, (1998) (PubMed).
Moynihan, Ardley, Leek, Thompson, Brindle, Markham, Robinson: "Characterization of a human ubiquitin-conjugating enzyme gene UBE2L3." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 7, Issue 7, pp. 520-5, (1996) (PubMed).
Nuber, Schwarz, Kaiser, Schneider, Scheffner: "Cloning of human ubiquitin-conjugating enzymes UbcH6 and UbcH7 (E2-F1) and characterization of their interaction with E6-AP and RSP5." in: The Journal of biological chemistry, Vol. 271, Issue 5, pp. 2795-800, (1996) (PubMed).
Robinson, Leek, Thompson, Carr, Bailey, Moynihan, Coletta, Lench, Markham: "A human ubiquitin conjugating enzyme, L-UBC, maps in the Alzheimer's disease locus on chromosome 14q24.3." in: Mammalian genome : official journal of the International Mammalian Genome Society, Vol. 6, Issue 10, pp. 725-31, (1996) (PubMed).
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2L3 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro.