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TYMS antibody (C-Term)

Name: TYMS antibody (C-Term)
SKU: ABIN1882144
Price: 451 USD
Availability: InStock

TYMS Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB20041 unconjugated

Western blot analysis of lysate from Jurkat cell line, using TYSY Antibody (C-term) (ABIN1882144 and ABIN2840937). (TYMS antibody (C-Term))

Flow cytometric analysis of WiDr cells using TYSY Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). (TYMS antibody (C-Term))

FACS

Confocal immunofluorescent analysis of TYSY Antibody (C-term) (ABIN1882144 and ABIN2840937) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (TYMS antibody (C-Term))

Formalin-fixed and paraffin-embedded human colon carcinoma with TYSY Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (TYMS antibody (C-Term))

IHC (p)

4 images

(3 references)

Catalog No. ABIN1882144

Datasheet as PDF

Target See all TYMS Antibodies

TYMS (Thymidylate Synthetase (TYMS))
Binding Specificity
15

12

6

5

3

3

3

1

1

1

1

1
AA 265-294, C-Term
Reactivity
91

29

14

3

2

2

2

1

1

1
Human
Host
48

42

1
Rabbit
Compatible Secondaries
Clonality
48

43
Polyclonal
Conjugate
45

7

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This TYMS antibody is un-conjugated
Application
50

41

33

22

19

19

18

16

14

13

8

7

6

3

3

2

2

1

1
Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen

This TYSY antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-294 amino acids from the C-terminal region of human TYSY.

Clone

RB20041

Isotype

Ig Fraction

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anti-Thymidylate Synthetase (TYMS) (AA 36-313) antibody
TYMS Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Thymidylate Synthetase (TYMS) (AA 60-174) antibody
TYMS Reactivity: Human WB, IF, FACS, IHC, ISt, RIA, StM Host: Mouse Monoclonal TYMS-1884 unconjugated

anti-Thymidylate Synthetase (TYMS) antibody
TYMS Reactivity: Human WB, IF, IHC (p), IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Thymidylate Synthetase (TYMS) (AA 111-210) antibody
TYMS Reactivity: Human WB, IF, ELISA, IHC (p) Host: Mouse Monoclonal 3A1 unconjugated

anti-Thymidylate Synthetase (TYMS) antibody
TYMS Reactivity: Human WB, IHC, IHC (p) Host: Mouse Monoclonal 1D6 unconjugated

anti-Thymidylate Synthetase (TYMS) antibody
TYMS Reactivity: Human WB, IHC, IHC (p) Host: Mouse Monoclonal 5H8 unconjugated

anti-Thymidylate Synthetase (TYMS) (AA 111-210) antibody
TYMS Reactivity: Human WB, ELISA, IHC (p), IP Host: Mouse Monoclonal 2B2 unconjugated

anti-Thymidylate Synthetase (TYMS) (AA 2-54) antibody
TYMS Reactivity: Human WB, IF, ELISA, IP Host: Rabbit Polyclonal unconjugated

anti-Thymidylate Synthetase (TYMS) antibody
TYMS Reactivity: Human, Mouse, Rat IF Host: Rabbit Polyclonal unconjugated

anti-Thymidylate Synthetase (TYMS) (AA 60-174) antibody
TYMS Reactivity: Human IF, FACS, ELISA, RIA, Coat Host: Mouse Monoclonal rTYMS-1884 unconjugated Recombinant Antibody

Application Notes

IF: 1:10~50. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Expiry Date

6 months
Ma, He, Zhang, Fei, Niu, Wang, Ding, Xu, Chen, Zhu: "DNA methylation-regulated miR-193a-3p dictates resistance of hepatocellular carcinoma to 5-fluorouracil via repression of SRSF2 expression." in: The Journal of biological chemistry, Vol. 287, Issue 8, pp. 5639-49, (2012) (PubMed).

Ren, Kim, Koh, Chang, Eom, Yi, Seong, Kim, Bronner, Cho: "Comparative analysis of thymidylate synthase at the protein, mRNA, and DNA levels as prognostic markers in colorectal adenocarcinoma." in: Journal of surgical oncology, Vol. 100, Issue 7, pp. 546-52, (2009) (PubMed).

Schiffer, Clifton, Davisson, Santi, Stroud: "Crystal structure of human thymidylate synthase: a structural mechanism for guiding substrates into the active site." in: Biochemistry, Vol. 34, Issue 50, pp. 16279-87, (1996) (PubMed).
Target

TYMS (Thymidylate Synthetase (TYMS))

Abstract

TYMS Products

Background

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs.

Molecular Weight

35716

NCBI Accession

NP_001062

UniProt

P04818

Pathways

Mitotic G1-G1/S Phases