Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Predicted Reactivity
B, Zf
Purification
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen
This Cellular Apoptosis Susceptibility antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-84 amino acids from the N-terminal region of human Cellular Apoptosis Susceptibility.
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Expiry Date
6 months
Stella Tsai, Chen, Tung, Tsou, Tsao, Liao, Chen, Yeh, Yeh, Jiang: "Serum cellular apoptosis susceptibility protein is a potential prognostic marker for metastatic colorectal cancer." in: The American journal of pathology, Vol. 176, Issue 4, pp. 1619-28, (2010) (PubMed).
Uen, Tai, Shen, Lee, Tsao, Deng, Chiou, Hsu, Hsieh, Liao, Jiang: "Differential distributions of CSE1L/CAS and E-cadherin in the polarized and non-polarized epithelial glands of neoplastic colorectal epithelium." in: Journal of molecular histology, Vol. 41, Issue 4-5, pp. 259-66, (2010) (PubMed).
Tung, Tsai, Tung, Tsao, Chen, Yeh, Liao, Jiang: "Higher prevalence of secretory CSE1L/CAS in sera of patients with metastatic cancer." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 18, Issue 5, pp. 1570-7, (2009) (PubMed).
Goldberg, Alexander, Pellicena, Zhang, Tsuda, Miller: "Src phosphorylates Cas on tyrosine 253 to promote migration of transformed cells." in: The Journal of biological chemistry, Vol. 278, Issue 47, pp. 46533-40, (2003) (PubMed).
Behrens, Brinkmann, Wellmann: "CSE1L/CAS: its role in proliferation and apoptosis." in: Apoptosis : an international journal on programmed cell death, Vol. 8, Issue 1, pp. 39-44, (2003) (PubMed).
Jiang, Liao, Tai: "CAS/CSE 1 stimulates E-cadhrin-dependent cell polarity in HT-29 human colon epithelial cells." in: Biochemical and biophysical research communications, Vol. 294, Issue 4, pp. 900-5, (2002) (PubMed).
Wellmann, Flemming, Behrens, Wuppermann, Lang, Oldhafer, Pastan, Brinkmann: "High expression of the proliferation and apoptosis associated CSE1L/CAS gene in hepatitis and liver neoplasms: correlation with tumor progression." in: International journal of molecular medicine, Vol. 7, Issue 5, pp. 489-94, (2001) (PubMed).
Brinkmann, Brinkmann, Bera, Wellmann, Pastan: "Tissue-specific alternative splicing of the CSE1L/CAS (cellular apoptosis susceptibility) gene." in: Genomics, Vol. 58, Issue 1, pp. 41-9, (1999) (PubMed).
2610100P18Rik antibody, AA407533 antibody, Capts antibody, Cas antibody, Xpo2 antibody, cas antibody, chromosome segregation 1-like (S. cerevisiae) antibody, CSE1 chromosome segregation 1-like (yeast) antibody, Cse1l antibody, cse1l antibody
Background
Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex. After translocation, RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind. CSE1L binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition, CSE1L may play a role both in apoptosis and in cell proliferation.