This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This ZNF274 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 53-82 amino acids from the N-terminal region of human ZNF274.
ZNF274
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
WB: 1:1000
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Expiry Date
6 months
Wu, Ma, Brown, Geisler, Li, Tzeng, Jia, Jurisica, Li: "Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening." in: Proteomics, Vol. 7, Issue 11, pp. 1775-85, (2007) (PubMed).
Sripathy, Stevens, Schultz: "The KAP1 corepressor functions to coordinate the assembly of de novo HP1-demarcated microenvironments of heterochromatin required for KRAB zinc finger protein-mediated transcriptional repression." in: Molecular and cellular biology, Vol. 26, Issue 22, pp. 8623-38, (2006) (PubMed).
Casademunt, Carter, Benzel, Frade, Dechant, Barde: "The zinc finger protein NRIF interacts with the neurotrophin receptor p75(NTR) and participates in programmed cell death." in: The EMBO journal, Vol. 18, Issue 21, pp. 6050-61, (2000) (PubMed).
Yano, Ueki, Oda, Seki, Masuho, Muramatsu: "Identification and characterization of human ZNF274 cDNA, which encodes a novel kruppel-type zinc-finger protein having nucleolar targeting ability." in: Genomics, Vol. 65, Issue 1, pp. 75-80, (2000) (PubMed).
This gene encodes a zinc finger protein containing five C2H2-type zinc finger domains, one or two Kruppel-associated box A (KRAB A) domains, and a leucine-rich domain. The encoded protein has been suggested to be a transcriptional repressor. It localizes predominantly to the nucleolus. Alternatively spliced transcript variants encoding different isoforms exist. These variants utilize alternative polyadenylation signals.