This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This UBR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 245-274 amino acids from the N-terminal region of human UBR1.
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Expiry Date
6 months
Alkhouri, Kaplan, Kay, Shealy, Crowe, Bauhuber, Zenker: "Johanson-Blizzard syndrome with mild phenotypic features confirmed by UBR1 gene testing." in: World journal of gastroenterology : WJG, Vol. 14, Issue 44, pp. 6863-6, (2008) (PubMed).
Wei, Lin, Yang, Wang, Chang, Chen, Chen et al.: "Thiazolidinediones modulate the expression of beta-catenin and other cell-cycle regulatory proteins by targeting the F-box proteins of Skp1-Cul1-F-box protein E3 ubiquitin ligase independently of..." in: Molecular pharmacology, Vol. 72, Issue 3, pp. 725-33, (2007) (PubMed).
Target
UBR1
(Ubiquitin Protein Ligase E3 Component N-Recognin 1 (UBR1))
The N-end rule pathway is one proteolytic pathway of the ubiquitin system. The recognition component of this pathway, encoded by this gene, binds to a destabilizing N-terminal residue of a substrate protein and participates in the formation of a substrate-linked multiubiquitin chain. This leads to the eventual degradation of the substrate protein. The protein described in this record has a RING-type zinc finger and a UBR-type zinc finger. Mutations in this gene have been associated with Johanson-Blizzard syndrome.