T Antigen (AA 15-43), (N-Term) antibody

Catalog No. ABIN1881864

Product Details

Target: T Antigen

Binding Specificity: AA 15-43, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Predicted Reactivity: Chicken,Mouse (Murine)

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification. Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Immunogen: This T antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-43 amino acids from the N-terminal region of human T.

Clone: RB41833

Application Details

Application Notes: WB: 1:1000,IF: 1:10-50

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.39 mg/mL

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

References

Jambhekar, Rekhi, Thorat, Dikshit, Agrawal, Puri: "Revisiting chordoma with brachyury, a \new age\" marker: analysis of a validation study on 51 cases."" in: Archives of pathology & laboratory medicine, Vol. 134, Issue 8, pp. 1181-7, (2010) (PubMed).

Yang, Ng, Alcorta, Liebsch, Sheridan, Li, Goldstein, Parry, Kelley: "T (brachyury) gene duplication confers major susceptibility to familial chordoma." in: Nature genetics, Vol. 41, Issue 11, pp. 1176-8, (2009) (PubMed).

Bondue, Lapouge, Paulissen, Semeraro, Iacovino, Kyba, Blanpain: "Mesp1 acts as a master regulator of multipotent cardiovascular progenitor specification." in: Cell stem cell, Vol. 3, Issue 1, pp. 69-84, (2008) (PubMed).

Oakley, Fuhrer, Seethala: "Brachyury, SOX-9, and podoplanin, new markers in the skull base chordoma vs chondrosarcoma differential: a tissue microarray-based comparative analysis." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 21, Issue 12, pp. 1461-9, (2008) (PubMed).

Target Details

Target: T Antigen

Background: The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

Synonyms: T, Brachyury protein, Protein T

NCBI Accession: NP_003172, NP_001257413

UniProt: O15178