This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This SEMA7A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-202 amino acids from the N-terminal region of human SEMA7A.
Seltsam, Strigens, Levene, Yahalom, Moulds, Moulds, Hustinx, Weisbach, Figueroa, Bade-Doeding, DeLuca, Blasczyk: "The molecular diversity of Sema7A, the semaphorin that carries the JMH blood group antigens." in: Transfusion, Vol. 47, Issue 1, pp. 133-46, (2007) (PubMed).
Yamada, Kubo, Takeshita, Harashima, Kawano, Mine, Sagawa, Sugamura, Itoh: "Molecular cloning of a glycosylphosphatidylinositol-anchored molecule CDw108." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 7, pp. 4094-100, (1999) (PubMed).
Angelisová, Drbal, Cerný, Hilgert, Horejsí: "Characterization of the human leukocyte GPI-anchored glycoprotein CDw108 and its relation to other similar molecules." in: Immunobiology, Vol. 200, Issue 2, pp. 234-45, (1999) (PubMed).
Lange, Liehr, Goen, Gebhart, Fleckenstein, Ensser: "New eukaryotic semaphorins with close homology to semaphorins of DNA viruses." in: Genomics, Vol. 51, Issue 3, pp. 340-50, (1998) (PubMed).
Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes.