PROX1 antibody (AA 175-206)

Catalog No. ABIN1881686

Product Details anti-PROX1 Antibody

Target: PROX1 (Prospero Homeobox 1 (PROX1))

Binding Specificity: AA 175-206

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PROX1 antibody is un-conjugated

Application: Western Blotting (WB)

Predicted Reactivity: M

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This PROX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-206 amino acids from human PROX1.

Clone: RB15516

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-PROX1 antibody (ABIN1881686)

Nagai, Li, Hatano, Toshihito, Yuge, Ito, Utsumi, Saito, Kinoshita: "Mutations and aberrant DNA methylation of the PROX1 gene in hematologic malignancies." in: Genes, chromosomes & cancer, Vol. 38, Issue 1, pp. 13-21, (2003) (PubMed).

Hong, Harvey, Noh, Schacht, Hirakawa, Detmar, Oliver: "Prox1 is a master control gene in the program specifying lymphatic endothelial cell fate." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 225, Issue 3, pp. 351-7, (2002) (PubMed).

Petrova, Mäkinen, Mäkelä, Saarela, Virtanen, Ferrell, Finegold, Kerjaschki, Ylä-Herttuala, Alitalo: "Lymphatic endothelial reprogramming of vascular endothelial cells by the Prox-1 homeobox transcription factor." in: The EMBO journal, Vol. 21, Issue 17, pp. 4593-9, (2002) (PubMed).

Mouta Carreira, Nasser, di Tomaso, Padera, Boucher, Tomarev, Jain: "LYVE-1 is not restricted to the lymph vessels: expression in normal liver blood sinusoids and down-regulation in human liver cancer and cirrhosis." in: Cancer research, Vol. 61, Issue 22, pp. 8079-84, (2001) (PubMed).

Target Details for PROX1

Target: PROX1 (Prospero Homeobox 1 (PROX1))

Alternative Name: PROX1 (PROX1 Products)

Synonyms: xprox1 antibody, prospero antibody, PROX1 antibody, LOC100232600 antibody, A230003G05Rik antibody, prox1 antibody, zgc:111888 antibody, prospero homeobox 1 antibody, prospero homeobox 1 L homeolog antibody, prospero homeobox 1a antibody, PROX1 antibody, prox1.L antibody, prox1 antibody, Prox1 antibody, prox1a antibody

Background: The expression pattern of Prox1 suggests that it has a role in a variety of embryonic tissues, including lens. Prox mRNA is present in many different human tissues with lens demonstrating the highest level. Homozygous Prox1-null mice die at midgestation from multiple developmental defects, and a targeted effect on lens development has been reported. Prox1 inactivation caused abnormal cellular proliferation, downregulated expression of the cell cycle inhibitors Cdkn1b and Cdkn1c, misexpression of E-cadherin, and excessive apoptosis. Consequently, mutant lens cells failed to polarize and elongate properly, resulting in a hollow lens. Prox1 is expressed in a subpopulation of endothelial cells that by budding and sprouting give rise to the lymphatic system. Prox1 appears to be a specific and required regulator of the development of the lymphatic system. Prox1 also has been documented to be required for hepatocyte migration in the mouse. Loss of Prox1 results in a smaller liver with a reduced population of clustered hepatocytes. The homeodomain protein Prox1 regulates the egress of progenitor cells from the cell cycle in the embryonic mouse retina. Cells lacking Prox1 are less likely to stop dividing, and ectopic expression of Prox1 forces progenitor cells to exit the cell cycle. Prox1 acts as a key participant in progenitor-cell proliferation and cell-fate determination in the vertebrate retina.

Molecular Weight: 83203

NCBI Accession: NP_001257545, NP_002754

UniProt: Q92786

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation