MBNL1 antibody (C-Term)

Catalog No. ABIN1881533

Product Details anti-MBNL1 Antibody

Target: MBNL1 (Muscleblind-like Protein 1 (MBNL1))

Binding Specificity: AA 255-284, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MBNL1 antibody is un-conjugated

Application: Western Blotting (WB)

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This MBNL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 255-284 amino acids from the C-terminal region of human MBNL1.

Clone: RB42214

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-MBNL1 antibody (ABIN1881533)

Sen, Talukdar, Liu, Tam, Reddy, Webster: "Muscleblind-like 1 (Mbnl1) promotes insulin receptor exon 11 inclusion via binding to a downstream evolutionarily conserved intronic enhancer." in: The Journal of biological chemistry, Vol. 285, Issue 33, pp. 25426-37, (2010) (PubMed).

Arambula, Ramisetty, Baranger, Zimmerman: "A simple ligand that selectively targets CUG trinucleotide repeats and inhibits MBNL protein binding." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 38, pp. 16068-73, (2009) (PubMed).

Cardani, Baldassa, Botta, Rinaldi, Novelli, Mancinelli, Meola: "Ribonuclear inclusions and MBNL1 nuclear sequestration do not affect myoblast differentiation but alter gene splicing in myotonic dystrophy type 2." in: Neuromuscular disorders : NMD, Vol. 19, Issue 5, pp. 335-43, (2009) (PubMed).

Holt, Jacquemin, Fardaei, Sewry, Butler-Browne, Furling, Brook, Morris: "Muscleblind-like proteins: similarities and differences in normal and myotonic dystrophy muscle." in: The American journal of pathology, Vol. 174, Issue 1, pp. 216-27, (2009) (PubMed).

Teplova, Patel: "Structural insights into RNA recognition by the alternative-splicing regulator muscleblind-like MBNL1." in: Nature structural & molecular biology, Vol. 15, Issue 12, pp. 1343-51, (2008) (PubMed).

Target Details for MBNL1

Target: MBNL1 (Muscleblind-like Protein 1 (MBNL1))

Alternative Name: MBNL1 (MBNL1 Products)

Synonyms: MBNL1 antibody, zgc:153954 antibody, Mbnl antibody, mKIAA0428 antibody, EXP antibody, EXP35 antibody, EXP40 antibody, EXP42 antibody, MBNL antibody, muscleblind like splicing regulator 1 antibody, muscleblind-like splicing regulator 1 antibody, muscleblind like splicing factor 1 antibody, MBNL1 antibody, mbnl1 antibody, Mbnl1 antibody

Background: Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues.

Molecular Weight: 41817

NCBI Accession: NP_066368, NP_997175, NP_997176, NP_997177, NP_997178, NP_997179, NP_997180

UniProt: Q9NR56

Pathways: Ribonucleoprotein Complex Subunit Organization