ErbB2/Her2 antibody (pTyr1248)

Catalog No. ABIN1881305

Product Details anti-ErbB2/Her2 Antibody

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Binding Specificity: pTyr1248

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ErbB2/Her2 antibody is un-conjugated

Application: Western Blotting (WB), Dot Blot (DB)

Purification: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This ErbB2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1248 of human ErbB2.

Clone: RB40739

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:8000. WB: 1:8000. DB: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-ErbB2/Her2 antibody (ABIN1881305)

Brandt, Mikesch, Simon, Rötger, Kemming, Schier, Sauter, Bürger: "Selective expression of a splice variant of decay-accelerating factor in c-erbB-2-positive mammary carcinoma cells showing increased transendothelial invasiveness." in: Biochemical and biophysical research communications, Vol. 329, Issue 1, pp. 318-23, (2005) (PubMed).

Ghatak, Misra, Toole: "Hyaluronan constitutively regulates ErbB2 phosphorylation and signaling complex formation in carcinoma cells." in: The Journal of biological chemistry, Vol. 280, Issue 10, pp. 8875-83, (2005) (PubMed).

Provinciali, Re, Donnini, Orlando, Bartozzi, Di Stasio, Smorlesi: "Effect of resveratrol on the development of spontaneous mammary tumors in HER-2/neu transgenic mice." in: International journal of cancer. Journal international du cancer, Vol. 115, Issue 1, pp. 36-45, (2005) (PubMed).

Yee, Young, Rosol, Vanbuskirk, Clinton: "Dietary (n-3) polyunsaturated fatty acids inhibit HER-2/neu-induced breast cancer in mice independently of the PPARgamma ligand rosiglitazone." in: The Journal of nutrition, Vol. 135, Issue 5, pp. 983-8, (2005) (PubMed).

Beckers, Herrmann, Rieger, Drobyshev, Horsch, Hrabé de Angelis, Seliger: "Identification and validation of novel ERBB2 (HER2, NEU) targets including genes involved in angiogenesis." in: International journal of cancer. Journal international du cancer, Vol. 114, Issue 4, pp. 590-7, (2005) (PubMed).

Target Details for ErbB2/Her2

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Alternative Name: ERBB2 (ErbB2/Her2 Products)

Synonyms: CD340 antibody, HER-2 antibody, HER-2/neu antibody, HER2 antibody, MLN 19 antibody, NEU antibody, NGL antibody, TKR1 antibody, Erbb-2 antibody, Neu antibody, c-erbB2 antibody, c-neu antibody, mKIAA3023 antibody, wu:fv70f10 antibody, zgc:63601 antibody, erb2 antibody, erb-b2 receptor tyrosine kinase 2 antibody, ERBB2 antibody, Erbb2 antibody, erbb2 antibody

Background: ErbB2 is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors.

Molecular Weight: 137910

NCBI Accession: NP_001005862, NP_004439

UniProt: P04626

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development