CRYAA antibody (AA 77-106)

Catalog No. ABIN1881229

Product Details anti-CRYAA Antibody

Target: CRYAA (Crystallin, alpha A (CRYAA))

Binding Specificity: AA 77-106

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRYAA antibody is un-conjugated

Application: Western Blotting (WB)

Predicted Reactivity: B, Hs, M, Pig, Rb, Rat, Sh

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This CRYAA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-106 amino acids from the Central region of human CRYAA.

Clone: RB28544

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-CRYAA antibody (ABIN1881229)

Deng, Chen, Xie, Zhao, Gong, Liu, Zhang, Sun, Liu, Ma, Batra, Li: "The small heat shock protein alphaA-crystallin is expressed in pancreas and acts as a negative regulator of carcinogenesis." in: Biochimica et biophysica acta, Vol. 1802, Issue 7-8, pp. 621-31, (2010) (PubMed).

Li, Yang, Ma, Zhang, Zhang, Wang, Zhu: "Autosomal dominant congenital nuclear cataracts caused by a CRYAA gene mutation." in: Current eye research, Vol. 35, Issue 6, pp. 492-8, (2010) (PubMed).

Pang, Su, Feng, Tang, Gu, Zhang, Ma, Yan: "Effects of congenital cataract mutation R116H on alphaA-crystallin structure, function and stability." in: Biochimica et biophysica acta, Vol. 1804, Issue 4, pp. 948-56, (2010) (PubMed).

Bhagyalaxmi, Padma, Reddy, Reddy: "Association of G>A transition in exon-1 of alpha crystallin gene in age-related cataracts." in: Oman journal of ophthalmology, Vol. 3, Issue 1, pp. 7-12, (2010) (PubMed).

Target Details for CRYAA

Target: CRYAA (Crystallin, alpha A (CRYAA))

Alternative Name: CRYAA (CRYAA Products)

Synonyms: CRYA1 antibody, CTRCT9 antibody, HSPB4 antibody, AI323437 antibody, Acry-1 antibody, Crya-1 antibody, Crya1 antibody, DAcry-1 antibody, lop18 antibody, alpha-a-crystallin antibody, cryaa-A antibody, CRYAA antibody, wu:fc06h04 antibody, zgc:92036 antibody, HspB4 antibody, crystallin alpha A antibody, crystallin, alpha A antibody, alphaA-crystallin antibody, alpha-crystallin A chain-like antibody, crystallin alpha A S homeolog antibody, alpha-crystallin A chain antibody, CRYAA antibody, Cryaa antibody, CpipJ_CPIJ005646 antibody, cryaa antibody, LOC100384978 antibody, cryaa.S antibody, LOC458589 antibody, LOC102724652 antibody

Background: Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families, beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone, instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits, the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed, alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC). [provided by RefSeq].

Molecular Weight: 19909

NCBI Accession: NP_000385

UniProt: P02489

Pathways: M Phase