Calpain 1+2 antibody
CAPN1+CAPN2
Reactivity: Cow, Human
EIA, IP, WB
Host: Mouse
Monoclonal
28F3
unconjugated
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- Target
- Calpain 1+2 (CAPN1+CAPN2)
- Reactivity
- Cow, Human
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
- Specificity
- Species Reactivity: Reacts with human and bovine calpain, but does not recognize rat or mouse calpain.
- Immunogen
- Balb/C mice were immunized with calpain purified from human placenta. The resultant hybridomas were selected for reactivity with the immunogen by ELISA and with the antigen by Western blot.
- Clone
- 28F3
- Isotype
- IgG1
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- Application Notes
- ELISA: Use at 0.5-1 µg/ml. Immunoprecipitation: Use at 1-2 µg/ml. Western blot: 0.5-1 µg/ml The optimal dilution for a specific application should be determined by the researcher.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Monoclonal antibody against human Calpain I, II (small subunit). This antibody is available at a concentration of 100 µg/100 µl in 0.02 M sodum phosphate, pH 7.5, 0.15 M sodium chloride, 50% glycerol, 3 mM sodium azide. This antibody was purified to >95
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
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- Target
- Calpain 1+2 (CAPN1+CAPN2)
- Alternative Name
- Calpain (CAPN1+CAPN2)
- Background
- The calpains are calcium-dependent cysteine proteases that are widely expressed in mammalian systems. Both m-calpain (calpain II) and u-calpain (calpain I) are composed of an 80 kDa subunit. Whereas the 30 kDa subunit is shared by both enzymes, the larger catalytic subunits are different and exhibit the distinct Ca++ requirements that are suggested by their names. Whereas m-calpain requires milli-molar levels of calcium, u-calpain is active at micromolar concentrations of Ca++. In addition to the ubiquitously expressed m- and u-calpains, some tissue-specific calpains have been identified. The calpains appear to serve multiple physiological roles, and ideas concerning the functions of these enzymes are in a state of rapid flux. Secificity: Clone 28F3 specifically recognizes the 28-30 kDa subunit of m-calpain and µ-calpain.
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