PUMA antibody
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- Target See all PUMA (BBC3) Antibodies
- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PUMA antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
- Purpose
- Polyclonal Antibody to p53 Upregulated Modulator Of Apoptosis (PUMA)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against PUMA. It has been selected for its ability to recognize PUMA in immunohistochemical staining and western blotting.
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- OVA Conjugated p53 Upregulated Modulator Of Apoptosis (PUMA)
- Isotype
- IgG
- Top Product
- Discover our top product BBC3 Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL Immunohistochemistry: 5-20 μg/mL Immunocytochemistry: 5-20 μg/mL Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
- Alternative Name
- p53 Upregulated Modulator Of Apoptosis (BBC3 Products)
- Synonyms
- BBC3 antibody, Puma antibody, JFY-1 antibody, JFY1 antibody, PUMA antibody, PUMA/JFY1 antibody, BCL2 binding component 3 antibody, Bcl-2 binding component 3 antibody, BBC3 antibody, Bbc3 antibody, bbc3 antibody
- Background
- JFY1, BBC3, BCL2 Binding Component 3
- Pathways
- p53 Signaling, Positive Regulation of Endopeptidase Activity
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