KRT20 antibody (AA 9-424)
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- Target See all KRT20 Antibodies
- KRT20 (Keratin 20 (KRT20))
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Binding Specificity
- AA 9-424
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This KRT20 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Polyclonal Antibody to Cytokeratin 20 (CK 20)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against CK 20. It has been selected for its ability to recognize CK 20 in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Pig
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Cytokeratin 20 (CK 20) corresdonding to His9~Ile424 with N-terminal His Tag
- Isotype
- IgG
- Top Product
- Discover our top product KRT20 Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- KRT20 (Keratin 20 (KRT20))
- Alternative Name
- Cytokeratin 20 (KRT20 Products)
- Synonyms
- KRT20 antibody, CD20 antibody, CK-20 antibody, CK20 antibody, K20 antibody, KRT21 antibody, Krt21 antibody, 9030623C06Rik antibody, keratin 20 antibody, KRT20 antibody, Krt20 antibody
- Background
- KRT20, Keratin 20, Protein IT, Keratin, type I cytoskeletal 20
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