BNIP3 antibody (AA 3-134)
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- Target See all BNIP3 Antibodies
- BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))
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Binding Specificity
- AA 3-134
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Reactivity
- Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This BNIP3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Polyclonal Antibody to Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against BNIP3. It has been selected for its ability to recognize BNIP3 in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Human, Mouse, Pig
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) corresdonding to Gln3~Ala134 with N-terminal His and GST Tag
- Isotype
- IgG
- Top Product
- Discover our top product BNIP3 Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL
Immunohistochemistry: 5-20 μg/mL
Immunocytochemistry: 5-20 μg/mL
Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))
- Alternative Name
- Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3 Products)
- Synonyms
- NIP3 antibody, Nip3 antibody, BCL2 interacting protein 3 antibody, BCL2/adenovirus E1B interacting protein 3 antibody, BNIP3 antibody, Bnip3 antibody
- Background
- NIP3
- Pathways
- Autophagy, Brown Fat Cell Differentiation
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