AF4 antibody (AA 1-183)
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- Target See all AF4 (AFF1) products
- AF4 (AFF1) (AF4/FMR2 Family, Member 1 (AFF1))
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Binding Specificity
- AA 1-183
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This AF4 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against AFF1. It has been selected for its ability to recognize AFF1 in immunohistochemical staining and western blotting.
- Purification
- Antigen-specific affinity chromatography
- Immunogen
- AFF1 (Met1-Gly183)
- Isotype
- IgG
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- Application Notes
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- Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5000 Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
- Expiry Date
- 12 months
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- Target
- AF4 (AFF1) (AF4/FMR2 Family, Member 1 (AFF1))
- Alternative Name
- AF4/FMR2 Family, Member 1 (AFF1 Products)
- Synonyms
- AF4 antibody, MLLT2 antibody, PBM1 antibody, 9630032B01Rik antibody, AW319193 antibody, Af4 antibody, Mllt2h antibody, Rob antibody, Mllt2 antibody, AF4/FMR2 family member 1 antibody, AF4/FMR2 family, member 1 antibody, AFF1 antibody, Aff1 antibody
- Background
- Alternative Names: AF-4, AF4, AF4-MLL, MLL/AF4, MLLT2, PBM1, Myeloid/Lymphoid Or Mixed-Lineage Leukemia, Translocated To 2, Pre-B-Cell Monocytic Leukemia Partner 1
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