This Triiodothyronine T3 antibody is conjugated to Biotin
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Specificity
The antibody is a rabbit polyclonal antibody raised against T3. It has been selected for its ability to recognize T3 in immunohistochemical staining and western blotting.
Purification
Protein A + Protein G affinity chromatography
Immunogen
The antibody is arabbit polyclonal antibody raised against T3 conjugated to biotin.
T3
Reactivity: Human
RIA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Western blotting: 0.2-2µg/mL;1:250-2500 Immunohistochemistry: 5-20µg/mL;1:25-100 Immunocytochemistry: 5-20µg/mL;1:25-100 Optimal working dilutions must be determined by end user.
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
Preservative
Sodium azide
Precaution of Use
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice
Avoid repeated freeze/thaw cycles
Storage
4 °C/-20 °C
Storage Comment
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.