HSP27 antibody (AA 1-201)
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- Target See all HSP27 Antibodies
- HSP27 (Heat Shock Protein 27 (HSP27))
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Binding Specificity
- AA 1-201
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Reactivity
- Cow
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This HSP27 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
- Purpose
- Polyclonal Antibody to Heat Shock Protein 27 (Hsp27)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against Hsp27. It has been selected for its ability to recognize Hsp27 in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Human, Mouse
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Heat Shock Protein 27 (Hsp27) corresdonding to Met1~Lys201 with N-terminal His Tag
- Isotype
- IgG
- Top Product
- Discover our top product HSP27 Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL
1:500-2000 Immunohistochemistry: 5-20 μg/mL
1:50-200 Immunocytochemistry: 5-20 μg/mL
1:50-200 Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- HSP27 (Heat Shock Protein 27 (HSP27))
- Alternative Name
- Heat Shock Protein 27 (HSP27 Products)
- Synonyms
- 27 antibody, 27K antibody, 28 antibody, CG4466 antibody, Dhsp27 antibody, DmHSP27 antibody, DmHsp27 antibody, Dmel\\CG4466 antibody, HSP27 antibody, HSP28 antibody, Hsp 27 antibody, Hsp28 antibody, anon-WO0140519.69 antibody, hsp 27 antibody, hsp-27 antibody, hsp26 antibody, hsp27 antibody, hsp27/26 antibody, hsp28 antibody, small hsp locus 67B antibody, Heat shock protein 27 antibody, heat shock protein 27 antibody, Hsp27 antibody, CpipJ_CPIJ005642 antibody
- Background
- HSPB1, HSP-B1, CMT2F, HSP28, Hsp25, Heat Shock 27 kDa Protein 1, 28 kDa heat shock protein, Heat shock protein beta-1, Estrogen-regulated 24 kDa protein,
- Pathways
- VEGF Signaling
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