TOP2 antibody (AA 1079-1328)

Catalog No. ABIN1860828

Product Details anti-TOP2 Antibody

Target: TOP2 (DNA Topoisomerase II (TOP2))

Binding Specificity: AA 1079-1328

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: The antibody is a rabbit polyclonal antibody raised against TOP2. It has been selected for its ability to recognize TOP2 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography

Immunogen: TOP2 (Gly1079-Ser1328)

Isotype: IgG

Application Details

Application Notes:

• Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5000 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for TOP2

Target: TOP2 (DNA Topoisomerase II (TOP2))

Alternative Name: Topoisomerase II (TOP2) (TOP2 Products)

Synonyms: Topoii antibody, ptr11 antibody, DDBDRAFT_0191045 antibody, DDBDRAFT_0231510 antibody, DDB_0191045 antibody, DDB_0231510 antibody, CG10223 antibody, CHRAC antibody, Dmel\\CG10223 antibody, TOP2 antibody, TOPO II antibody, TOPOII antibody, TopII antibody, Topo II antibody, TopoII antibody, dCHRAC antibody, dTopoII antibody, top2 antibody, topo II antibody, topoII antibody, topoisomerase II antibody, DNA topoisomerase II antibody, DNA topoisomerase 2 antibody, DNA topoisomerase ii antibody, type IIA topoisomerase; DNA gyrase topoisomerase IV B subunit antibody, Topoisomerase 2 antibody, TOP2 antibody, A583L antibody, top2 antibody, CNA03600 antibody, Tb09.160.4090 antibody, Tb11.01.3390 antibody, LMJF_28_2280 antibody, Top2 antibody

Background: Alternative Names: DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme

Pathways: Mitotic G1-G1/S Phases