NEXN antibody (AA 409-605)

Catalog No. ABIN1859994

Product Details anti-NEXN Antibody

Target: NEXN (Nexilin (NEXN))

Binding Specificity: AA 409-605

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEXN antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: MGHHHHHHSG SEF- EQ LRQEMGEEEE ENETFGLSRE YEELIKLKRS GSIQAKNLKS KFEKIGQLSE KEIQKKIEEE RARRRAIDLE IKEREAENFH EEDDVDVRPA RKSEAPFTHK VNMKARFEQM AKAREEEEQR RIEEQKLLRM QFEQREIDAA LQKKREEEEE EEGSIMNGST AEDEEQTRSG APWFKKPLKN TSVVDSEPVR FTVKV

Specificity: It has been selected for its ability to recognize NEXN in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: Recombinant NEXN expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against NEXN.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Comment:

Content: The quality control contains recombinant NEXN (Glu409~Val605) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for NEXN

Target: NEXN (Nexilin (NEXN))

Alternative Name: Nexilin (NEXN Products)

Synonyms: 1110046H09Rik antibody, AA553326 antibody, NELIN antibody, CMH20 antibody, nexilin antibody, nexilin F-actin binding protein antibody, nexilin F-actin binding protein S homeolog antibody, nexilin (F actin binding protein) antibody, LOC100422793 antibody, nexn antibody, Nexn antibody, nexn.S antibody, NEXN antibody