MT2 antibody (AA 1-62)

Catalog No. ABIN1859895

Product Details anti-MT2 Antibody

Target: MT2 (Metallothionein 2 (MT2))

Binding Specificity: AA 1-62

Reactivity: Rabbit

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This MT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: MGHHHHHHSG SEF- MDPNCSCAAA GDSCTCANSC TCKACKCTSC KKSCCSCCPP GCAKCAQGCI CKGASDKCSC CA

Specificity: It has been selected for its ability to recognize MT2 in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: MT2 (AA 1-62)

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Comment:

Content: The quality control contains recombinant MT2 (Met1~Ala62) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for MT2

Target: MT2 (Metallothionein 2 (MT2))

Abstract: MT2 Products

Synonyms: FGQTL2 antibody, MEL-1B-R antibody, MT2 antibody, Mt-2 antibody, MT-II antibody, AA409533 antibody, MT antibody, mt2b antibody, wu:fc77e11 antibody, wu:fj10c11 antibody, zgc:112061 antibody, MT-2 antibody, mt2 antibody, melatonin receptor 1B antibody, metallothionein 2 antibody, Metallothionein 2 antibody, metallothionein-2 antibody, MTNR1B antibody, Mt2 antibody, MT2 antibody, mt2 antibody, LOC101117955 antibody, LOC109048941 antibody, LOC102135658 antibody

Pathways: Transition Metal Ion Homeostasis