MIB1 antibody (AA 769-1000)
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- Target See all MIB1 Antibodies
- MIB1 (Mindbomb E3 Ubiquitin Protein Ligase 1 (MIB1))
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Binding Specificity
- AA 769-1000
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This MIB1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
- Purpose
- Polyclonal Antibody to Mindbomb Homolog 1 (MIB1)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against MIB1. It has been selected for its ability to recognize MIB1 in immunohistochemical staining and western blotting.
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Mindbomb Homolog 1 (MIB1) corresdonding to Arg769~Glu1000 with N-terminal His Tag
- Isotype
- IgG
- Top Product
- Discover our top product MIB1 Primary Antibody
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- Application Notes
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Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- MIB1 (Mindbomb E3 Ubiquitin Protein Ligase 1 (MIB1))
- Alternative Name
- Mindbomb Homolog 1 (MIB1 Products)
- Synonyms
- DIP-1 antibody, DIP1 antibody, LVNC7 antibody, MIB antibody, ZZANK2 antibody, ZZZ6 antibody, E430019M12Rik antibody, Mib antibody, mKIAA1323 antibody, dip-1 antibody, mib antibody, mindbomb antibody, cg5841 antibody, chunp6889 antibody, fe47f05 antibody, im:7148100 antibody, mib1 antibody, wu:fe47f05 antibody, CG5841 antibody, D-mib antibody, D-mib1 antibody, Dmel\CG5841 antibody, Dmib antibody, MIB1 antibody, Mib1 antibody, dmib1 antibody, l(3)72CDa antibody, l(3)72Db antibody, mib1/2 antibody, mind-bomb antibody, mindbomb E3 ubiquitin protein ligase 1 antibody, mindbomb E3 ubiquitin protein ligase 1 L homeolog antibody, mind bomb 1 antibody, MIB1 antibody, Mib1 antibody, mib1.L antibody, mib1 antibody
- Background
- DIP-1, MIB, ZZANK2, ZZZ6, Mind bomb homolog 1, DAPK-interacting protein 1, E3 ubiquitin-protein ligase MIB1, Zinc finger ZZ type with ankyrin repeat domain protein 2
- Pathways
- SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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