C-Jun N-Terminal Kinases (AA 145-419) antibody
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- Target
- C-Jun N-Terminal Kinases
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Binding Specificity
- AA 145-419
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
- Application
- Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
- Purpose
- Polyclonal Antibody to c-Jun N-terminal Kinase 1 (JNK1)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against JNK1. It has been selected for its ability to recognize JNK1 in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Chicken, Cow, Dog, Goat, Guinea Pig, Horse, Mouse, Pig, Rat, Sheep
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant c-Jun N-terminal Kinase 1 (JNK1) corresdonding to Ala145~Ala419 (Accession # P45983)
- Isotype
- IgG
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- Application Notes
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Western blotting: 0.5-2 μg/mL
1:180-700 Immunohistochemistry: 5-20 μg/mL
1:18-70 Immunocytochemistry: 5-20 μg/mL
1:18-70 Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- C-Jun N-Terminal Kinases
- Alternative Name
- c-Jun N-terminal Kinase 1
- Synonyms
- JNK antibody, JNK-46 antibody, JNK1 antibody, JNK1A2 antibody, JNK21B1/2 antibody, PRKM8 antibody, SAPK1 antibody, SAPK1c antibody, AI849689 antibody, Prkm8 antibody, mitogen-activated protein kinase 8 antibody, MAPK8 antibody, Mapk8 antibody
- Background
- MAPK8, JNK1A2, JNK21B1/2, PRKM8, SAPK1, JUN N-Terminal Kinase, Stress-activated protein kinase 1c, Mitogen Activated Protein Kinase 8
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