IL23 antibody (AA 22-196)

Catalog No. ABIN1859405

Product Details anti-IL23 Antibody

Target: IL23 (Interleukin 23 (IL23))

Binding Specificity: AA 22-196

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL23 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Sequence: MGHHHHHHSG SEF- VPRSSSPDW AQCQQLSRNL CTLAWSAHTP VGQMDLLREE GEEETKSDVP RIQCGDGCDP QGLKDNSQFC LQRIRQGLVF YKHLLDSDIF TGEPSLLPDS PVDQLHTSLL GLSQLLQPED HHWETQQMPR LSPSQQWQRS LLRSKILRSL QAFLAIAARV FAHGAATLTE PLVPTA

Specificity: It has been selected for its ability to recognize IL23 in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: Recombinant IL23 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against IL23.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Comment:

Content: The quality control contains recombinant IL23 (Val22~Ala196) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for IL23

Target: IL23 (Interleukin 23 (IL23))

Alternative Name: Interleukin 23 (IL23 Products)

Synonyms: IL-23 antibody, p19 antibody, SGRF antibody, il23p19 antibody, interleukin 23, alpha subunit p19 antibody, interleukin 23 subunit alpha antibody, Il23a antibody, IL23A antibody