GPC4 antibody (AA 222-468)

Catalog No. ABIN1859053

Product Details anti-GPC4 Antibody

Target: GPC4 (Glypican 4 (GPC4))

Binding Specificity: AA 222-468

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPC4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: The antibody is a rabbit polyclonal antibody raised against GPC4. It has been selected for its ability to recognize GPC4 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography

Immunogen: GPC4 (Ala222-Leu468)

Isotype: IgG

Application Details

Application Notes:

• Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5000 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for GPC4

Target: GPC4 (Glypican 4 (GPC4))

Abstract: GPC4 Products

Synonyms: K-glypican antibody, knypek antibody, xgly4 antibody, Xgly4 antibody, glypican-4 antibody, 9530073D23Rik antibody, AF311610 antibody, AI385680 antibody, AI661372 antibody, chunp6920 antibody, gpc4, antibody, gpc4/6 antibody, gpc6 antibody, kny antibody, wu:fc47a08 antibody, wu:fe05f10 antibody, glypican 4 antibody, glypican 4 L homeolog antibody, GPC4 antibody, gpc4.L antibody, gpc4 antibody, Gpc4 antibody

Background: Alternative Names: K-Glypican, Glypican Proteoglycan 4, Secreted glypican-4

Pathways: Glycosaminoglycan Metabolic Process