ENPP1 antibody (AA 568-793)

Catalog No. ABIN1858719

Product Details anti-ENPP1 Antibody

Target: ENPP1 (Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1))

Binding Specificity: AA 568-793

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENPP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: The antibody is a rabbit polyclonal antibody raised against ENPP1. It has been selected for its ability to recognize ENPP1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography

Immunogen: ENPP1 (Glu568-Ile793)

Isotype: IgG

Application Details

Application Notes:

• Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5000 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

References for anti-ENPP1 antibody (ABIN1858719)

Liu, Wang, Hou, He, Ma, Ye, Yu, Chen, Wang, Zhang: "Chondrocyte-derived exosomes promote cartilage calcification in temporomandibular joint osteoarthritis." in: Arthritis research & therapy, Vol. 24, Issue 1, pp. 44, (2022) (PubMed).

Target Details for ENPP1

Target: ENPP1 (Ectonucleotide Pyrophosphatase/phosphodiesterase 1 (ENPP1))

Abstract: ENPP1 Products

Synonyms: ENPP1 antibody, si:ch211-142e24.2 antibody, zgc:136238 antibody, ARHR2 antibody, M6S1 antibody, NPP1 antibody, NPPS antibody, PC-1 antibody, PCA1 antibody, PDNP1 antibody, 4833416E15Rik antibody, AI428932 antibody, C76301 antibody, CD203c antibody, E-NPP1 antibody, Ly-41 antibody, Npps antibody, Pca antibody, Pca-1 antibody, Pdnp1 antibody, ttw antibody, twy antibody, Pc1 antibody, ectonucleotide pyrophosphatase/phosphodiesterase 1 antibody, ectonucleotide pyrophosphatase/phosphodiesterase 1 L homeolog antibody, ENPP1 antibody, enpp1 antibody, enpp1.L antibody, Enpp1 antibody

Background: Alternative Names: M6S1, NPP1, NPPS, PC-1, PCA1, PDNP1, Membrane component chromosome 6 surface marker 1, Alkaline phosphodiesterase I, Plasma-cell membrane glycoprotein PC-1

Pathways: Regulation of Carbohydrate Metabolic Process