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CA12 antibody (AA 161-318)

CA12 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1858202
  • Target See all CA12 Antibodies
    CA12 (Carbonic Anhydrase 12 (CA12))
    Binding Specificity
    • 15
    • 8
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 161-318
    Reactivity
    • 48
    • 8
    • 7
    Mouse
    Host
    • 37
    • 10
    • 2
    Rabbit
    Clonality
    • 40
    • 9
    Polyclonal
    Conjugate
    • 26
    • 6
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CA12 antibody is un-conjugated
    Application
    • 30
    • 22
    • 13
    • 13
    • 13
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Purpose
    Polyclonal Antibody to Carbonic Anhydrase XII (CA12)
    Specificity
    The antibody is a rabbit polyclonal antibody raised against CA12. It has been selected for its ability to recognize CA12 in immunohistochemical staining and western blotting.
    Cross-Reactivity
    Human, Rat
    Purification
    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
    Immunogen
    Recombinant Carbonic Anhydrase XII (CA12)
    Isotype
    IgG
    Top Product
    Discover our top product CA12 Primary Antibody
  • Application Notes

    Western blotting: 0.5-2 μg/mL

    1:500-2000 Immunohistochemistry: 5-20 μg/mL

    1:50-200 Immunocytochemistry: 5-20 μg/mL

    1:50-200 Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions
    For Research Use only
  • Validation #100909 (Immunohistochemistry)
    'Independent Validation' Badge
    by
    School of Clinical Sciences, University of Bristol
    No.
    #100909
    Date
    02/21/2017
    Antigen
    CA12
    Lot Number
    A20170109527
    Method validated
    Immunohistochemistry
    Positive Control
    mouse kidney, mouse ciliary body
    Negative Control
    secondary antibody only
    Notes
    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    ABIN3204699
    Secondary Antibody
    goat anti-rabbit IgG Alexa Fluor 488 antibody (Life Technology, A-11006, lot 1348202)
    Full Protocol
    • Cryo-section:
      • Wash mouse eye and kidney tissue in PBS.
      • Cryo-protection. Suspend tissue in Optimum Cutting Temperature (OCT) embedding media (Thermo Scientific, Lot 03801137).
      • Gently freeze suspended tissue in liquid nitrogen for 5min.
      • Sections performed on a cryostat (LEICA, CM3050S) set to 12µm.
      • Fix sections in 4% paraformaldehyde (PFA) for 5min at room temperature.
      • Rinse sections in PBS 3 times for 5min.
      • Block sections with 5% normal goat serum for 1h at RT.
    • Immunostaining:
      • Incubate sections with primary rabbit-anti CA12 antibody (antibodies-online, ABIN3204699, lot A20170109527) diluted 1:100 in PBSA, overnight at 4°C. Include a negative control without primary antibody and a kidney positive control.
      • Rinse sections with PBS 3 times for 5min. Keep negative control in a separate jar.
      • Incubate sections with secondary goat anti-rabbit IgG Alexa Fluor 488 antibody conjugate (Life Technology, A-11006, lot 1348202) diluted 1:500 in 1% Bovine Serum Albumin in PBS (PBSA) buffer for 2h at RT.
      • Counterstain with DAPI in the mounting medium step.
    • Wash and mount:
      • Rinse sections in PBS 3 times for 5min.
      • Mount sections in Vectorshield mounting medium with DAPI (Vector laboratories, H-1200, lot ZC0814).
    • Microscopy:
      • Image acquisition on an EVOS FL Color fluorescence and transmitted light microscope (Life technologies, Cat no. AMEFC4300) at 20x magnification.
    Experimental Notes
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
    Preservative
    ProClin
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    Expiry Date
    24 months
  • Target
    CA12 (Carbonic Anhydrase 12 (CA12))
    Alternative Name
    Carbonic Anhydrase XII (CA12 Products)
    Synonyms
    CAXII antibody, HsT18816 antibody, CAR12 antibody, 2310047E01Rik antibody, AI314958 antibody, Ca12 antibody, ca12 antibody, carbonic anhydrase 12 antibody, carbonic anhydrase XII L homeolog antibody, CA12 antibody, Car12 antibody, ca12.L antibody
    Background
    CA-XII, CAXII, Carbonate dehydratase XII, Tumor antigen HOM-RCC-3.1.3
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