FEZ1 antibody (Internal Region)

Catalog No. ABIN185737

Product Details anti-FEZ1 Antibody

Target: FEZ1 (Fasciculation and Elongation Protein zeta 1 (Zygin I) (FEZ1))

Binding Specificity: Internal Region

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This FEZ1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: FEZ1 / Zygin 1

Sequence: QNKQKEQREL MKK

Specificity: This antibody is expected to recognize isoform 1 (NP_005094.1) only.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-QNKQKEQRELMKK, from the internal region of the protein sequence according to NP_005094.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5-8 μg/mL.

Western Blot: Approx 75 kDa band observed in lysates of cell line Kelly, ans in Rat Brain lysates (calculated MW of 45.1 kDa according to human NP_005094.1 and 45.2 kDa according to Rat NP_112328.1). This molecular weight is routinely observed by other sour

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FEZ1

Target: FEZ1 (Fasciculation and Elongation Protein zeta 1 (Zygin I) (FEZ1))

Alternative Name: FEZ1 (FEZ1 Products)

Synonyms: UNC-76 antibody, UNC76 antibody, wu:fj14c12 antibody, zgc:73258 antibody, fez1 antibody, xfez1 antibody, fasciculation and elongation protein zeta 1 antibody, fasciculation and elongation protein zeta 1 (zygin I) antibody, fasciculation and elongation protein zeta 1 L homeolog antibody, FEZ1 antibody, Fez1 antibody, fez1 antibody, fez1.L antibody

Background: FEZ1, zygin 1, fasciculation and elongation protein zeta 1 (zygin I)

Gene ID: 9638, 235180, 81730

NCBI Accession: NP_005094