CPT1A antibody (Internal Region)

Catalog No. ABIN185710

Product Details anti-CPT1A Antibody

Target: CPT1A (Carnitine Palmitoyltransferase 1A (Liver) (CPT1A))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CPT1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: CPT1A

Sequence: DPAQTVEQRL KLFK

Specificity: This antibody is expected to recognize both reported isoforms (NP_001867.2, NP_001027017.1) but is not expected to recognise CPT1B.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-DPAQTVEQRLKLFK, from the internal region of the protein sequence according to NP_001867.2, NP_001027017.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Liver. Recommended concentration: 5-7 μg/mL.

Western Blot: Approx 80-85 kDa band observed in lysates of cell line MCF7 (calculated MW of 86.2 kDa according to NP_001027017.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CPT1A

Target: CPT1A (Carnitine Palmitoyltransferase 1A (Liver) (CPT1A))

Alternative Name: CPT1A (CPT1A Products)

Background: CPT1A, carnitine palmitoyltransferase 1A (liver) , CPT1, CPT1-L, L-CPT1 , carnitine palmitoyltransferase 1A, carnitine palmitoyltransferase I, carnitine palmitoyltransferase I, liver

Gene ID: 1374

NCBI Accession: NP_001867, NP_001027017

Pathways: AMPK Signaling, Regulation of Lipid Metabolism by PPARalpha, Feeding Behaviour, Monocarboxylic Acid Catabolic Process