FXR1 antibody (Internal Region)

Catalog No. ABIN185683

Product Details anti-FXR1 Antibody

Target: FXR1 (Fragile X Mental Retardation, Autosomal Homolog 1 (FXR1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This FXR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: FXR1

Sequence: RIEGDNENKL PRED

Specificity: This antibody is expected to recognise all reported isoforms (NP_005078.2, NP_001013456.1 and NP_001013457.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-RIEGDNENKLPRED, from the internal region of the protein sequence according to NP_005078.2, NP_001013456.1, NP_001013457.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 75+80 kDa band observed in lysates of cell line NIH-3T3 (calculated MW of 59.9 kDa according to NP_001013457.1). The observed molecular weight corresponds to earlier findings in literature (Khandjian et al Hum Mol Genet. 1998 Dec,7(13

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for FXR1

Target: FXR1 (Fragile X Mental Retardation, Autosomal Homolog 1 (FXR1))

Alternative Name: FXR1 (FXR1 Products)

Synonyms: FXR1P antibody, wu:fb16f11 antibody, wu:fd18c10 antibody, zgc:66226 antibody, Fxr1h antibody, Fxr1p antibody, XtFxr1p antibody, fxr1h antibody, xFxr1p antibody, FXR1 antibody, 1110050J02Rik antibody, 9530073J07Rik antibody, AI851072 antibody, xfxr1 antibody, fxr1 antibody, FMR1 autosomal homolog 1 antibody, fragile X mental retardation, autosomal homolog 1 antibody, fragile X mental retardation gene 1, autosomal homolog antibody, FMR1 autosomal homolog 1 L homeolog antibody, FMR1 autosomal homolog 1 S homeolog antibody, FXR1 antibody, fxr1 antibody, Fxr1 antibody, fxr1.L antibody, fxr1.S antibody

Background: FXR1, fragile X mental retardation, autosomal homolog 1 , fragile X mental retardation-related protein 1

Gene ID: 8087, 14359, 361927

NCBI Accession: NP_005078, NP_001013456, NP_001013457