UNC13D antibody (Internal Region)

Catalog No. ABIN185677

Product Details anti-UNC13D Antibody

Target: UNC13D (Unc-13 Homolog D (UNC13D))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This UNC13D antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: Munc13-4 / UNC13D (Internal)

Sequence: ETQKHKKDLH PLFD

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: C-ETQKHKKDLHPLFD

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Thymus shows cytoplasm staining in select areas. Recommended concentration: 2.5 μg/mL. In paraffin embedded Mouse Lung shows staining of the resperatory epithelium in wildtype but not in the knock-out. Data

Western Blot: Approx. 105 kDa band observed in Human T-lymphocyte and HeLa lysates (calculated MW of 123 kDa according to NP_954712.1). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for UNC13D

Target: UNC13D (Unc-13 Homolog D (UNC13D))

Alternative Name: UNC13D (UNC13D Products)

Synonyms: FHL3 antibody, HLH3 antibody, HPLH3 antibody, Munc13-4 antibody, UNC13D antibody, 2610108D09Rik antibody, Jinx antibody, mFLJ00067 antibody, Unc13h4 antibody, BIGH3 antibody, CDB1 antibody, CDG2 antibody, CDGG1 antibody, CSD antibody, CSD1 antibody, CSD2 antibody, CSD3 antibody, EBMD antibody, LCD1 antibody, unc-13 homolog D antibody, unc-13 homolog D (C. elegans) antibody, transforming growth factor beta induced antibody, UNC13D antibody, Unc13d antibody, TGFBI antibody, unc13d antibody, un13d antibody

Background: UNC13D, Munc13-4, unc-13 homolog D (C. elegans) , FHL3, HLH3, HPLH3, unc-13 homolog D

Gene ID: 201294, 70450, 192177

NCBI Accession: NP_954712

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process