Serotonin Receptor 3A antibody (Internal Region)

Catalog No. ABIN185673

Product Details anti-Serotonin Receptor 3A Antibody

Target: Serotonin Receptor 3A (HTR3A)

Binding Specificity: Internal Region

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 3A antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Purpose: Serotonin receptor 3A / HTR3A

Sequence: GPQDFEKSPR DR

Specificity: This antibody is expected to recognise all three reported isoforms

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-GPQDFEKSPRDR, from the internal region of the protein sequence according to NP_998786.2, NP_000860.2, NP_001155244.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Small Intestine. Recommended concentration: 3.75 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunocytochemistry:This product has been successfully used in ICC on Rat: Lu CL et al, Gastroenterology. 2009 Sep,137(3):1040-50. PMID: 19344720.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended co

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Serotonin Receptor 3A antibody (ABIN185673)

Lu, Hsieh, Dun, Oprea, Wang, Luo, Lin, Chang, Lee: "Estrogen rapidly modulates 5-hydroxytrytophan-induced visceral hypersensitivity via GPR30 in rats." in: Gastroenterology, Vol. 137, Issue 3, pp. 1040-50, (2009) (PubMed).

Target Details for Serotonin Receptor 3A

Target: Serotonin Receptor 3A (HTR3A)

Alternative Name: HTR3A (HTR3A Products)

Synonyms: MGC52789 antibody, HTR3A antibody, 5-HT3 antibody, 5HT3R antibody, HTR3 antibody, 5-HT-3 antibody, 5-HT3A antibody, 5-HT3R antibody, 5HT3 antibody, Htr3 antibody, 5-hydroxytryptamine (serotonin) receptor 3A, ionotropic L homeolog antibody, 5-hydroxytryptamine receptor 3A antibody, 5-hydroxytryptamine (serotonin) receptor 3A antibody, htr3a.L antibody, HTR3A antibody, Htr3a antibody

Background: HTR3A, serotonin receptor, 5-hydroxytryptamine (serotonin) receptor 3A, 5-HT-3, 5-HT3A, 5-HT3R, 5HT3R, HTR3, 5-hydroxytryptamine (serotonin) receptor-3, 5HT3 serotonin receptor, Serotonin-gated ion channel receptor, truncated receptor, containing only 3 t

Gene ID: 3359

NCBI Accession: NP_998786, NP_000860, NP_001155244

Pathways: Synaptic Membrane