Patched 1 antibody (Internal Region)

Catalog No. ABIN185667

Product Details anti-Patched 1 Antibody

Target: Patched 1 (PTCH1)

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Patched 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose: PTCH (Internal)

Sequence: HPESRHHPPS NPRQQ

Specificity: This antibody is expected to recognise all four reported isoforms (NP_000255.2, NP_001077072.1, NP_001077071.1, NP_001077075.1). Reported variants represent identical protein (NP_001077075.1, NP_001077076.1, NP_001077073.1, NP_001077074.1).

Cross-Reactivity: Cow, Dog, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-HPESRHHPPSNPRQQ, from the internal region of the protein sequence according to NP_000255.2, NP_001077072.1, NP_001077071.1, NP_001077075.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 150 kDa band observed in Human Brain lysates (calculated MW of 153.9 kDa according to NP_001077071.1). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence :Expression of the protein seen in the Golgi/membrane of NIH3T3 cells. Recommended concentration: 5μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-Patched 1 antibody (ABIN185667)

Gerardo Valadez, Grover, Carter, Calcutt, Abiria, Lundberg, Williams, Cooper: "Identification of Hedgehog pathway responsive glioblastomas by isocitrate dehydrogenase mutation." in: Cancer letters, Vol. 328, Issue 2, pp. 297-306, (2012) (PubMed).

Target Details for Patched 1

Target: Patched 1 (PTCH1)

Alternative Name: PTCH1 (PTCH1 Products)

Background: PTCH, patched homolog (Drosophila), RP11-435O5.3, BCNS, FLJ42602, HPE7, NBCCS, PTC, PTC1, PTCH1 , PTCH protein +12b, PTCH protein +4', PTCH protein -10, patched, patched (Drosophila) homolog

Gene ID: 5727

NCBI Accession: NP_000255, NP_001077072, NP_001077071, NP_001077075

Pathways: Hedgehog Signaling, Carbohydrate Homeostasis, Tube Formation