PRNP antibody (Internal Region)

Catalog No. ABIN185661

Product Details anti-PRNP Antibody

Target: PRNP (Prion Protein (PRNP))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PRNP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Prion Protein (143-153)

Sequence: SDYEDRYYRE N

Specificity: Reported variants represent identical protein (NP_000302.1, NP_898902.1, NP_001073590.1, NP_001073591.1, NP_001073592.1).

Cross-Reactivity: Cow, Human, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-SDYEDRYYREN, from the internal region of the protein sequence according to NP_000302.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 25 kDa band observed in Human Brain lysates (calculated MW of 27.7 kDa according to NP_000302.1 and NP_898902.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PRNP

Target: PRNP (Prion Protein (PRNP))

Alternative Name: PRNP (PRNP Products)

Background: PRNP , prion protein (p27-30) (Creutzfeldt-Jakob disease, Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia) , ASCR, CD230, CJD, GSS, MGC26679, PRIP, PrP, PrP27-30, PrP33-35C, PrPc , CD230 antigen, major prion protein, prion protein, prion p

Gene ID: 5621, 19122, 24686

NCBI Accession: NP_000302

Pathways: Transition Metal Ion Homeostasis, Activated T Cell Proliferation