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Hsc70 antibody (Internal Region)

Name: Hsc70 antibody (Internal Region)
Brand: antibodies-online
SKU: ABIN185659
Price: 482.37 USD
Availability: InStock

Verified HSPA8 Reactivity: Human, Mouse WB, IHC, ELISA, IF Host: Goat Polyclonal unconjugated

ABIN185659 (0. (Hsc70 antibody (Internal Region))

ABIN185659 (2µg/ml) staining of A431 cell lysate (35µg protein in RIPA buffer). (Hsc70 antibody (Internal Region))

ABIN185659 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. (Hsc70 antibody (Internal Region))

3 images

(1 reference)

Catalog No. ABIN185659

Datasheet as PDF

Target See all Hsc70 (HSPA8) Antibodies

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
Binding Specificity
14

7

6

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Internal Region
Reactivity
83

48

43

21

17

14

13

11

11

11

10

10

5

5

5

4

4

3

3

3

2

1

1

1

1

1

1

1
Human, Mouse
Host
72

28

7

1
Goat
Compatible Secondaries
Clonality
68

39
Polyclonal
Conjugate
60

19

11

3

3

1

1

1

1

1

1

1

1

1

1

1

1
This Hsc70 antibody is un-conjugated
Application
94

50

40

30

29

28

16

14

11

11

11

5

4

2

2

2

2
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Purpose

HSPA8 (Isoform 1)

Sequence

EKLQGKINDE DKQK

Specificity

This antibody is expected to recognise isoform 1 (NP_006588.1) only.

Cross-Reactivity

Dog, Human, Mouse, Rat

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade

Verified

Immunogen

Peptide with sequence C-EKLQGKINDEDKQK, from the internal region of the protein sequence according to NP_006588.1.

Isotype

IgG

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HSPA8 Reactivity: Human, Rat, Mouse, Cow, Sheep, Pig, Zebrafish (Danio rerio), Goat WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 534-615) antibody
HSPA8 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 5 μg/mL.
Western Blot: Approx 75 kDa band observed in lysates of cell lines HepG2 and NIH3T3 (calculated MW of 70.9 kDa according to Human NP_006588.1 and Mouse NP_112442.2). This molecular weight is routinely observed by other sources. Recommended concentration:
Peptide ELISA: antibody detection limit dilution 1:4000.

Comment

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10μg/ml

Restrictions

For Research Use only
Format

Liquid

Concentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Minimize freezing and thawing.

Storage

-20 °C

Storage Comment

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Johnson, Kaplitt: "Novel mitochondrial substrates of omi indicate a new regulatory role in neurodegenerative disorders." in: PLoS ONE, Vol. 4, Issue 9, pp. e7100, (2009) (PubMed).
Target

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Alternative Name

HSPA8 (HSPA8 Products)

Background

HSPA8 , heat shock 70 kDa protein 8 , HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP, MGC131511, MGC29929, NIP71 , LPS-associated protein 1, N-myristoyltransferase inhibitor protein 71, constitutive heat shock protein 70, heat shock 70kD protein 8, heat sh

Gene ID

3312, 15481, 24468

NCBI Accession

NP_006588