Chromogranin A antibody (Internal Region)
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- Target See all Chromogranin A (CHGA) Antibodies
- Chromogranin A (CHGA)
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Chromogranin A antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Western Blotting (WB), ELISA
- Purpose
- Chromogranin A precursor
- Sequence
- QGAKERAHQQ KKH
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-QGAKERAHQQKKH, from the internal region of the protein sequence according to NP_001266.1.
- Isotype
- IgG
- Top Product
- Discover our top product CHGA Primary Antibody
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- Application Notes
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Western Blot: Approx 50 kDa band observed in lysates of cell lines Jurkat and LNCaP, and in Human Cerebellum, Pancreas and Kidney lysates. A band at 35 kDa was also observed in pancreas lysate (calculated MW of 50.7 kDa according to NP_001266.1 isoform 1 a
Peptide ELISA: antibody detection limit dilution 1:128000.
- Comment
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Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- Chromogranin A (CHGA)
- Alternative Name
- CHGA (CHGA Products)
- Background
- Chromogranin A precursor, chromogranin A (parathyroid secretory protein 1) , CHGA, CGA , parathyroid secretory protein 1, Vasostatin, Pancreastatin, Parastatin, Pituitary secretory protein I, SP1
- Gene ID
- 1113
- NCBI Accession
- NP_001266, NP_001288619
- Pathways
- Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling
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