AIRE antibody (Internal Region)

Catalog No. ABIN185643

Product Details anti-AIRE Antibody

Target: AIRE (Autoimmune Regulator (AIRE))

Binding Specificity: Internal Region

Reactivity: Human, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This AIRE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: AIRE

Sequence: KDVDLSQPRK GRKP

Cross-Reactivity: Cow, Dog, Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-KDVDLSQPRKGRKP, from the internal region of the protein sequence according to NP_000374.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Thymus shows nuclear staining in select thymocytes. This antibody has been successfully used in IHC on Human: Ströbel P et al. (2014) PMID: 24236644. This antibody has been successfully used in IHC on Pig:

Western Blot: Appr. 60 kDa in transfected HEK293 lysates (calculated MW of 57.7 kDa according to NP_000374.1). Recommended concentration: 0.5-1.0 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-AIRE antibody (ABIN185643)

Suzuki, Suzuki, Nakai, Sembon, Fuchimoto, Onishi: "Transcriptional and histological analyses of the thymic developmental process in the fetal pig." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 63, Issue 2, pp. 215-25, (2014) (PubMed).

Ströbel, Hartmann, Rosenwald, Kalla, Ott, Friedel, Schalke, Kasahara, Tomaru, Marx: "Corticomedullary differentiation and maturational arrest in thymomas." in: Histopathology, Vol. 64, Issue 4, pp. 557-66, (2014) (PubMed).

Target Details for AIRE

Target: AIRE (Autoimmune Regulator (AIRE))

Alternative Name: AIRE (AIRE Products)

Background: AIRE, autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy) , AIRE1, APECED, APS1, APSI, PGA1 , autoimmune regulator (APECED protein), autoimmune regulator (automimmune polyendocrinopathy candidiasis ectodermal dystrophy),

Gene ID: 326

NCBI Accession: NP_000374

Pathways: Chromatin Binding