MAP3K7 antibody (Internal Region)

Catalog No. ABIN185640

Product Details anti-MAP3K7 Antibody

Target: MAP3K7 (Mitogen-Activated Protein Kinase Kinase Kinase 7 (MAP3K7))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This MAP3K7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Purpose: TAK1 / MAP3K7

Sequence: AELDQDEKDQ QNT

Specificity: This antibody is expected to recognise isoform A (NP_003179.1) and isoform B (NP_663304.1).

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-AELDQDEKDQQNT, from the internal region of the protein sequence according to NP_003179.1, NP_663304.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 75 kDa band observed in lysates of cell lines HeLa, Daudi and NIH3T3, and approx. 80 kDa in lysates of cell line U937 (calculated MW of 67.2 according to Human NP_663304.1 and Mouse NP_033342.1). This molecular weight is routinely obse

Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MAP3K7

Target: MAP3K7 (Mitogen-Activated Protein Kinase Kinase Kinase 7 (MAP3K7))

Alternative Name: MAP3K7 (MAP3K7 Products)

Background: MAP3K7 , TAK1, mitogen-activated protein kinase kinase kinase 7 , TGF1a , TGF-beta activated kinase 1, transforming growth factor-beta-activated kinase 1

Gene ID: 6885, 26409

NCBI Accession: NP_003179, NP_663304

Pathways: NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Tube Formation, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway